Figure 2.
Relative product formation by APE1 with various mismatch combinations adjacent to THF. (a) Schematic of the mismatch substrates used in the study, where NO denotes the nucleotide opposite the APE1 active site and NA represents the 5′ nucleotide adjacent to THF. (b) A representative gel of three replicate assays shows the relative APE1 endonuclease activity with a G·G, AO·GA, A·A, T·T, TO·CA, C·C and AO·CA mismatch base pair placed directly 5′ to the THF. The substrate and product locations are indicated next to the denaturing gel image, with an asterisk denoting the 6-FAM label used for quantification. The average percentage of product formation is shown as the mean ± SEM, with N = 3.