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. 2018 Feb 21;218(3):1061–1075. doi: 10.1111/nph.15063

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© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PACLOBUTRAZOL RESISTANCE 1 (PRE1) expression associates with spinnable cotton fiber formation. (a) Phylogenetic analysis of PRE genes in Theobroma cacao (Thecc), Arabidopsis thaliana (At), Gossypium raimondii (Gorai) and G. hirsutum (Gh) by the maximum‐likelihood on nucleotide sequences. Subfamilies are denoted by different colors, with PRE1 being in the red clade. GhPRE1A/D, Gh_A05G3166_5/Gh_D04G0454; GhPRE2A/D, Gh_A12G1523/Gh_D12G1645; PRE3A/D, Gh_A03G0864/Gh_D02G1245; PRE4A/D, Gh_A07G1964/Gh_D07G2183; PRE5A/D, Gh _A09G0192/Gh_D09G0182; PRE6A/D, Gh_A03G0577/Gh_D03G0859; PRE7A/D, Gh_A05G2637/Gh_D05G2928; PRE8A/D, Gh_A07G1960/Gh_D07G2177; PRE9A/D, Gh_A09G0190/Gh_D09G0180; PRE10A/D, Gh_A11G3000/Gh_D11G0327; PRE11A/D, Gh_A11G2998/Gh_D11G0328; PRE12A/D, Gh_D12G1740/Gh_A12G1600; PRE13A/D, Gh_A13G0641/>Gh_D13G0759. The PRE ortholog from Amborella trichopoda (XM_006827104) was used as outgroup. (b) Expression of GhPRE genes in cotton fibers at different numbers of days post‐anthesis (DPA), as indicated, GhPRE1 is the major PRE gene expressed in cotton fiber. (c) Expression of PRE1 orthologs in fibers of the A‐genome species G. arboreum compared to two D‐genome species: left, G. raimondii, the model progenitor diploid to allopolyploid cottons, and right, G. thurberi, a phylogenetically more distant D‐genome species. (d) Expression of PRE1 orthologs in the A‐genome G. arboreum fibers during the fast elongation stage. The transcripts were analyzed by quantitative reverse transcription PCR (qRT‐PCR); error bars indicate SD (n = 3). (e) Expression levels of GhPRE1 in domesticated G. hirsutum cultivars and wild relatives, based on transcriptome data of 10‐DPA fiber. RPKM, reads per kilobase per million mapped reads.