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Deletion of all of the subunits of COMPASS results in a defect in silencing of gene expression at telomeres. The nonessential subunits of COMPASS (CPS60, 50, 40, 30, and 25) were deleted in the URA3 telomeric reporter strain ucc1001. Five-fold serial dilutions from 5 × 104 − 500 cells were applied to SD-ura (Left) and SD complete plate (Right). After 48 h, growth was compared with that of parental stain ucc1001.
