FIGURE 6.

Fisetin inhibits PI3K/AKT/GSK-3β signaling pathway and reverses EMT in vivo. Xenograft tumor metastasis was established by subcutaneous injection of MDA-MB-231 cells in the presence or absence of fisetin (100 mg/kg). (A) AKT activation was evaluated by immunohistochemistry staining. (B) Representative images of PTEN stained section of tumor tissues isolated from nude mice bearing breast cancer. (C) Vimentin and Snail in primary tumor tissues were examined by immunofluorescence assay. (D) PTEN and p-Akt and p-GSK-3β protein in the primary tumor tissues was examined by western blot. (E) EMT molecule markers were determined by western blot. The results are shown as the mean ± SD of six experiments, ^∗P < 0.05, ^∗∗P < 0.01 compared with control.