FIG. 2.

Manual Processing of lipoaspirate to yield SVF. In Photo 1, lipoaspirate is shown before mechanical breakdown using scissors (Photo 2). This breakdown provides a greater surface area on which the chemical digestion enzyme collagenase can act (Photo 3). After a defined time period of digestion, the resulting slurry is centrifuged, yielding a solution of three key parts as shown in Photo 4: a top layer of buoyant adipocytes, a middle layer of collagenase and remaining tumescent fluids, and a cellular pellet at the bottom containing SVF—a heterogeneous mix of various cell types. Color images available online at www.liebertpub.com/teb