FIG. 2.

Q-RT-PCR quantification of Stat3 mRNA levels. DCs were treated for 48 or 72 h with MIS416, MIS416-SS-Control_siRNA, and MIS416-SS-Stat3_siRNA using previously published sequences for Stat3-targeting siRNAs [27]. Analysis by Q-RT-PCR was performed on cDNA generated from total RNA extracted from treated cells. Downregulation of Stat3 mRNA levels occurs at 48 and 72 h in all samples treated with MIS416-SS-Stat3_siRNA compared with controls. The relative level of expression of Stat3 was set to 1 for MIS416-SS-Control_siRNA samples. The relative quantification was carried out using BioGazelle qBase+software. Error bars represent SEM. Results designated with ns were not significant. Results designated with * were significant (P < 0.05). This experiment was repeated three times.