FIG. 5.

Representative immunohistochemistry images and stain quantification of Bone Marrow constructs, Cord Blood constructs, and positive and negative native tissue controls. For each tissue section, a low magnification image is presented on the left with higher magnification images corresponding to the black boxed regions on the right. Dashed lines define construct edges. Scale bars are presented in the top image of each column and reflect the scale for all images in the column: 200 μm for low magnification images, 50 μm for high magnification and Control images. The percentage of the tissue section that showed DAB staining was quantified and presented as percent area stained for each antibody (right). Student's t-test was performed using p < 0.05 as statistical significance; starred bars show statistical significance between groups. Equine tendon was used as a positive control and equine articular cartilage as a negative control for collagen type I staining (Col I). Bone Marrow constructs had higher levels of staining for this marker of fibrocartilage. Equine articular cartilage was a positive control, and equine tendon was a negative control for collagen type II staining (Col II). Cord Blood constructs possessed higher levels of stain for this hyaline cartilage marker. Osteoarthritic equine articular cartilage served as a positive control, while nonarthritic equine articular cartilage served as a negative control for collagen type X (Col X). Bone Marrow constructs had higher stain uptake for this marker of hypertrophic cartilage.