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. 2018 Mar 2;26(4):963–975. doi: 10.1016/j.ymthe.2018.01.020

Figure 2.

Figure 2

Quantitative Quality of IS Formed by 4-1BB-CAR and CD28-CAR T Cells

(A) Confocal microscopy of Kappa-4-1BB-CAR T cells and Kappa-CD28-CAR T cells on a lipid bilayer carrying human Kappa IgG1-Alexa Fluor 647 (red). Fixed and permeabilized CAR T cells were stained for pZeta (cyan) and F-actin (magenta). A visual cartoon of the lipid bilayer and CAR T cells is displayed (left). Scale bars represent 25.0 μm. (B) Quantification of IS on the lipid bilayer-T cell focal plane by measuring the MFIs of F-actin, pZeta, and Kappa cluster on the lipid bilayers containing Kappa IgG1. Error bars show ±SD. The p value is for unpaired t-test. Data represent three independent experiments.