Figure 3.

Representative Images of Three-Dimensional Ultrasound Reconstructions of Abdominal Aorta, Photographs of Vessel Isolations, Immunohistochemistry, and Martius Scarlet Blue Demonstrate Profound Effect of VCAM-1-Targeted miR-Carrying Microbubbles

(A) 3D ultrasound reconstruction of abdominal aorta shows vessel lumen (in red), as well as massive areas of plaque buildup and aneurysm (in blue), from animals treated with Targ^MB-A₁₂₆ or Targ^MB-S₁₂₆, but not in animals treated with Targ^MB-M_126. (B) Vessel isolation shows clean abdominal aorta in mice treated with Targ^MB-M₁₂₆ but plaque buildup and aneurysms in mice given Targ^MB-S₁₂₆ or Targ^MB-A₁₂₆. (C) Immunohistochemistry confirmed a decrease in VCAM-1 expression for Targ^MB-M₁₂₆-treated animals as compared to those treated with Targ^MB-A₁₂₆ or Targ^MB-S₁₂₆. (D) Martius Scarlet Blue showed plaque buildup and aneurysm in abdominal arteries of Targ^MB-A₁₂₆- or Targ^MB-S₁₂₆-treated animals, whereas very little plaque buildup was observed in Targ^MB-M₁₂₆-treated mice.