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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Int J Radiat Biol. 2017 Nov;93(11):1257–1266. doi: 10.1080/09553002.2017.1377360

Figure 6.

Figure 6.

Effect of NADPH oxidase (NOX) inhibitors and NOX-2 neutralizing antibody on radiation-induced ROS generation in mouse brain. An increase in ROS generation in mouse brain 4 h after fractionated whole brain irradiation (FIR) was markedly and significantly attenuated in the presence of NOX inhibitors (FIR + APO or FIR + DPI) or NOX-2 neutralizing antibody (FIR + anti-NOX-2). (A) Sham-irradiation (Control); (B) 4 h after fractioned whole brain irradiation (FIR); (C) FIR + 1 mM apocynin (APO); (D) FIR + 20 μM diphenylene iodonium (DPI); (E) FIR + anti-NOX-2 antibody; (F) Quantitative analysis of fluorescence intensity. Data represents mean ± SEM for each group (n=4). **p<0.001 compared to control, #p<0.05; ##p<0.001 compared to irradiated brain (FIR). Scale bar: 100 μm.