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Detection of single base mismatches (red) in DNA duplexes by monitoring the DNA CT yield.48 The redox signal of a redox-active DNA-intercalating molecule, methylene blue (MB), is amplified via redox cycling with Fe(CN)63−/4− that oxidizes the reduced form of MB, leucomethylene blue (LB), in solution. Chronocoulometry (right) is used to quantify the attenuation with each intervening mismatch.
