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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: ACS Chem Biol. 2018 Jun 1;13(7):1799–1809. doi: 10.1021/acschembio.8b00347

Figure 4.

Figure 4.

Two-electrode setup for detecting DNMT1 in physiological solutions.69 Current is measured by a reporting electrode (top, gray) near the DNA-modified electrode, via charge transferred by Fe(CN)63− that can then electrocatalytically regenerate methylene blue that was reduced via DNA CT. Active DNMT1 is able to methylate a hemimethylated DNA substrate. Incubation with a methylation-dependent restriction enzyme leads to cleavage of DNA that was not exposed to active DNMT1, thereby decreasing the current measured by the reporting electrode. DNA that is methylated by DNMT1 will retain its structure and retain a high measured current.