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. 2018 Feb 28;6:1804. Originally published 2017 Oct 5. [Version 2] doi: 10.12688/f1000research.12252.2

Figure 6. Means and standard deviations of the phenotype of HSV-1 infected Vero cells.

Figure 6.

BFA was added to monolayers at 5, 8, 12 or 16 hpi (MOI of 5) and incubated until 20 hpi. For control, inoculated cells were incubated for 20 h without addition of BFA. Cells were rapidly frozen at 20 hpi and processed for electron microscopy. The phenotypes of envelopment were counted in 10 cellular profiles of 5 independent experiments: Capsids budding at the INM (B-INM), at the ONM and ER membranes (B-ONM/ER) and at the Golgi complex (B-Golgi); virions in the PNS-ER compartment (PNS-ER); virions derived by wrapping (Wrapp); virions in Golgi cisternae or large vacuoles (Golgi); capsids in the cytoplasmic matrix (capsids).