FIGURE 3.

Pharmacological DAGL inhibition impairs fear extinction. (A) Schematic diagram of the experimental paradigm. (B) (Far left panel) % freezing by C57BL/6J male mice during acquisition of cue-conditioned fear. (Middle panels) % freezing during auditory cue by mice during extinction training days 2 and 3 when D034 (50 mg kg⁻¹) was injected IP 2 h prior to trial. (Far right panel) % freezing during extinction recall (n = 17 DO34-treated male mice, n = 18 vehicle-treated male mice). (C) (Far left panel) % freezing by C57BL/6J female mice during acquisition of cue-conditioned fear when DO34 (50 mg kg⁻¹) was injected IP 2 h prior to trial. (Middle panels) % freezing during auditory cue during extinction training days 2 and 3. (Far right panel) % freezing during extinction recall (n = 18 DO34-treated female mice n = 20 vehicle-treated female mice). F- and P-values and η² for main effects of drug treatment shown in each panel. All values are presented as mean ± SEM.