Table 4.
Drug threshold values for GAP MCB parasites
| Anti-malarial drug | Test 1 | Test 2 | Test 3 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | Test sample | Control | Test sample | Control | Test sample | ||||
| 3D7 | W2 | GAP MCB | 3D7 | W2 | GAP MCB | 3D7 | W2 | GAP MCB | |
| CQ (chloroquine) | |||||||||
| IC50 (nM) | 14.85 | 206.8 | 14.37 | 16.53 | 297.6 | 13.77 | |||
| 95% confidence intervals (nM) | (8.489–25.98) | (131.6–325.0) | (8.671–23.80) | (9.880–27.64) | (179.3–493.9) | (8.149–23.28) | |||
| DHA (dihydroartemisinin) | |||||||||
| IC50 (nM) | 5.624 | 4.354 | 4.931 | 7.366 | 2.019 | 7.943 | |||
| 95% confidence intervals (nM) | (4.271–7.406) | (2.902–6.532) | (3.667–6.629) | (5.600–9.689) | (1.539–2.649) | (6.065–10.40) | |||
| PQ (piperaquine) | |||||||||
| IC50 (nM) | 18.15 | 46.02 | 15.27 | 30.69 | 48.85 | 23.59 | |||
| 95% confidence intervals (nM) | (10.41–31.66) | (29.78–71.11) | (8.490–27.47) | (19.33–48.72) | (34.94–68.31) | (15.29–36.39) | |||
| LF (lumefantrine) | |||||||||
| IC50 (nM) | No results | 70.35 | No results | No results | 42.32 | No results | 315.2 | 25.31 | 335.8 |
| 95% confidence intervals (nM) | (46.35–106.8) | (33.26–53.85) | (205.2–484.3) | (18.11–35.38) | (251.3–448.7) | ||||
| AQM (desethylamodiaquine) | |||||||||
| IC50 (nM) | 10.19 | 66.83 | 10.35 | 18.46 | 83.88 | 15.55 | |||
| 95% confidence intervals (nM) | (6.031–17.21) | (40.88–109.3) | (5.551–19.31) | (10.06–33.86) | (48.10–146.3) | (9.139–26.47) | |||
| ATQ (atovaquone) | |||||||||
| IC50 (nM) | 0.9587 | 1.939 | 1.147 | 0.7122 | 1.524 | 1.216 | |||
| 95% confidence intervals (nM) | (0.8493–1.082) | (1.752–2.147) | (0.9806–1.342) | (0.6224–0.8150) | (1.304–1.781) | (1.094–1.352) | |||
| PD (pyronaridine) | |||||||||
| IC50 (nM) | 5.398 | 9.897 | 7.804 | 27.67 | 21.88 | 12.59 | |||
| 95% confidence intervals (nM) | (3.359–8.675) | (5.615–17.45) | (3.473–17.54) | (15.88–48.19) | (11.82–40.49) | (6.720–23.57) | |||
| MQ (mefloquine HCl) | |||||||||
| IC50 (nM) | 101.5 | 15.32 | 98.87 | 157 | 12.26 | 61.27 | |||
| 95% confidence intervals (nM) | (72.61–141.8) | (10.85–21.65) | (69.17–141.3) | (79.04–311.8) | (8.573–17.53) | (43.67–85.96) | |||
| QN (quinine HCl) | |||||||||
| IC50 (nM) | 203.2 | 795.6 | 227.4 | 335.6 | 569.6 | 89.73 | |||
| 95% confidence intervals (nM) | (169.9–243.0) | (527.6–1200) | (173.1–298.8) | (195.2–577.1) | (432.1–750.9) | (62.87–128.1) | |||
Drug threshold values are expressed as mean IC50 (95% confidence interval) in nmol/L
The assay was repeated twice and reproducible results were obtained
A third assay was performed for lumefantrine as IC50 values were not detectable in the first two assays