Table 2.

Light microscopy findings by infection group and time point compared to uninfected NC group

	2 hpi			24 hpi			48 hpi			72 hpi
	EH	EL	CI	EH	EL	CI	EH	EL	CI	EH	EL	CI
Attached macrophages	+++	+++	+++	+++	+++	++	+++	+++	++	++	++	+
Macrophage vacuolization	-	-	+	++	+	++	+	+	+++	++	++	+++
Free Eta sporozoites	+	+	+	-	-	-	-	-	-	-	-	-
Intracellular Et a sporozoites	+	+	+	+	+	+	-	-	-	-	-	-
Intracellular meronts	-	-	-	+	+	++	++	++	++	++	++	++
Et a merozoites	-	-	-	-	-	-	-	-	-	+	+	-c
	TH	TL	CI	TH	TL	CI	TH	TL	CI	TH	TL	CI
Attached macrophages	+++	+++	+++	++	+++	++	+	++	++	-	+	+
Macrophage vacuolization	++	+	+	++	+	++	+++	++	+++	-	+++	+++
Free Tgb tachyzoites	+++	++	++	+	-	-	+++	+	+	+	+++	+++
Intracellular Tgb tachyzoites	+	+	+	++	+	+	+	++	++	+	++	+++

Findings were scored semiquantitatively (-, not observed; +, low amounts; ++, moderate amounts; +++, high amounts). Infection groups: TH, high-dose infection with T. gondii; TL, low-dose infection with T. gondii; EH, high-dose infection with E. tenella; EL, low-dose infection with E. tenella, CI co-infected group.

^aEt, E. tenella

^bTg, T. gondii

^cNot clearly observable due to the numerous replication of T. gondii tachyzoites presented in cell culture by light microscopy