After RAW264.7 cells were transfected miR-145-5p mimic and control, expression of OPG, RANKL, and RANK were detection at 4 time points. (A) Analysis of OPG, RANKL, and RANK mRNA expression via RT-PCR. (B) Analysis of OPG, RANKL, and RANK protein expression via Western blot. * Compared to 0 h, * P<0.05; ** P<0.01; & compared to 0 h, + P<0.05; && P<0.01; # compared to 0 h, # P<0.05; ## P<0.01; Values are means ±SD.