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RNA stability in phage lysates generated from different E. coli host strains. Radiolabeled U1hpII RNA (5 pmol) was mixed with either of two T7 phage lysates (5 × 109 pfu) prepared from E. coli BLT5615 or RNA5615 (an isogenic strain deficient in RNase I). After incubation at room temperature for the times indicated, RNA samples were analyzed by electrophoresis on a nondenaturing polyacrylamide gel.
