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. 2001 Oct 23;98(23):12954–12959. doi: 10.1073/pnas.211439598

Figure 5.

Figure 5

Suppression of a high affinity phage clone by the use of a competitor RNA. An equimolar mixture of recombinant phage displaying U1A or U2B" was diluted with a 106-fold excess of bare phage. U1hpIImut2 (50 nM) bearing a 3′ tail annealed to a biotinylated DNA oligonucleotide was used as the RNA bait. Wild-type U1hpII (0 or 25 nM) that had not been annealed to biotinylated DNA served as a competitor. All other conditions of in vitro selection were identical to those described for Fig. 3A. The Kd values previously determined for dissociation of U1hpIImut2 and U1hpII from U1A and U2B" are indicated (23).