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. 2018 Jul 19;131(14):jcs216580. doi: 10.1242/jcs.216580

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Microtubule depolymerization in scd1Δ cells leads to PORTLI growth. (A) Movie timepoints showing cell morphology and mCherry-Bgs4 distribution in the indicated genotypes, treated with 3-BrB-PP1 at −60 min and then with DMSO or MBC (plus 3-BrB-PP1). Diagrams show outlines at the beginning and end of movies. (B) Fields of cells as in A, after 3-BrB-PP1 and DMSO or MBC treatment for 300 min. (C) Quantification of mCherry-Bgs4 at cell tips during DMSO or MBC treatment (see Materials and Methods); ‘n’ indicates the number of cells scored. The difference between DMSO and MBC treatment was highly significant (P<0.0001; Fisher's exact test). Scale bars: 10 µm. See also Movie 1.