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. 2018 Jul 19;131(14):jcs216580. doi: 10.1242/jcs.216580

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Inhibition of Pom1 kinase activity in scd1Δ cells leads to PORTLI growth and randomized localization of Cdc42 GAP Rga4. (A) Movie timepoints showing cell morphology and distribution of Pom1-tdTomato or Pom1-as1-tdTomato and GFP-Bgs4 in the indicated genotypes after 3-BrB-PP1 treatment (added just after the 0 h timepoint). 3-BrB-PP1 inhibits both Cdc2-asM17 and Pom1-as1-tdTomato. Diagrams show outlines at the beginning and end of movies. (B) Quantification of GFP-Bgs4 at cell tips, from movies of the type in A. Differences were highly significant (P<0.0001; Fisher's exact test). (C) Single focal-plane movie timepoints showing cell morphology and distribution of Pom1-tdTomato or Pom1-as1-tdTomato and Rga4-3GFP in the indicated genotypes after 3-BrB-PP1 treatment. Scale bars: 10 µm. See also Figs S6 and S7 and Movies 7 and 8.