Table 5.
KEGG pathways | CTL | CP | ARGCP | P-value |
---|---|---|---|---|
MEMBRANE TRANSPORT | ||||
Transporters | 6.99 ± 1.20b | 6.18 ± 0.08a | 6.95 ± 0.09b | 0.001 |
ABC transporters | 3.32 ± 0.04 | 3.22 ± 0.06 | 3.28 ± 0.08 | 0.505 |
Secretion system | 1.41 ± 0.02 | 1.55 ± 0.03 | 1.44 ± 0.06 | 0.051 |
Phosphotransferase system (PTS) | 1.10 ± 0.13b | 0.75 ± 0.04a | 1.19 ± 0.04b | 0.004 |
CARBOHYDRATE METABOLISM | ||||
Amino sugar and nucleotide sugar metabolism | 1.58 ± 0.05b | 1.36 ± 0.03a | 1.60 ± 0.04b | 0.001 |
Glycolysis/Gluconeogenesis | 1.36 ± 0.03b | 1.22 ± 0.02a | 1.34 ± 0.02b | 0.002 |
Fructose and mannose metabolism | 1.16 ± 0.06ab | 1.04 ± 0.08a | 1.33 ± 0.07b | 0.033 |
Pyruvate metabolism | 1.10 ± 0.01a | 1.14 ± 0.01b | 1.11 ± 0.01a | 0.013 |
Starch and sucrose metabolism | 1.10 ± 0.04b | 0.86 ± 0.01a | 1.05 ± 0.02b | < 0.001 |
Pentose phosphate pathway | 0.91 ± 0.01c | 0.78 ± 0.01a | 0.88 ± 0.01b | < 0.001 |
Butanoate metabolism | 0.61 ± 0.02a | 0.90 ± 0.02c | 0.75 ± 0.01b | < 0.001 |
Galactose metabolism | 0.75 ± 0.05b | 0.56 ± 0.02a | 0.73 ± 0.02b | 0.001 |
Propanoate metabolism | 0.52 ± 0.02a | 0.79 ± 0.01c | 0.60 ± 0.01b | < 0.001 |
AMINO ACID METABOLISM | ||||
Amino acid related enzymes | 1.36 ± 0.01 | 1.32 ± 0.01 | 1.35 ± 0.02 | 0.151 |
Arginine and proline metabolism | 1.01 ± 0.03a | 1.09 ± 0.01b | 0.98 ± 0.02a | 0.006 |
Alanine, aspartate and glutamate metabolism | 0.86 ± 0.01 | 0.89 ± 0.01 | 0.87 ± 0.02 | 0.333 |
Cysteine and methionine metabolism | 0.83 ± 0.01 | 0.81 ± 0.01 | 0.84 ± 0.01 | 0.251 |
Glycine, serine and threonine metabolism | 0.67 ± 0.01a | 0.83 ± 0.01c | 0.70 ± 0.004b | < 0.001 |
Lysine biosynthesis | 0.67 ± 0.01 | 0.65 ± 0.01 | 0.67 ± 0.02 | 0.380 |
REPLICATION and REPAIR | ||||
DNA repair and recombination proteins | 2.89 ± 0.03b | 2.59 ± 0.02a | 2.82 ± 0.04b | < 0.001 |
Chromosome | 1.59 ± 0.01b | 1.44 ± 0.02a | 1.56 ± 0.02b | < 0.001 |
DNA replication proteins | 1.17 ± 0.02b | 1.01 ± 0.01a | 1.14 ± 0.02b | < 0.001 |
Homologous recombination | 0.92 ± 0.01c | 0.79 ± 0.01a | 0.88 ± 0.01b | < 0.001 |
Mismatch repair | 0.81 ± 0.01b | 0.70 ± 0.01a | 0.78 ± 0.01b | < 0.001 |
ENERGY METABOLISM | ||||
Oxidative phosphorylation | 1.05 ± 0.06a | 1.18 ± 0.02b | 0.98 ± 0.02a | 0.008 |
Methane metabolism | 1.06 ± 0.02 | 1.06 ± 0.01 | 1.04 ± 0.02 | 0.452 |
Carbon fixation pathways in prokaryotes | 0.82 ± 0.01a | 0.97 ± 0.01c | 0.84 ± 0.01b | < 0.001 |
Photosynthesis proteins | 1.12 ± 0.17b | 0.57 ± 0.07a | 0.76 ± 0.07a | 0.011 |
Photosynthesis | 0.94 ± 0.13b | 0.50 ± 0.05a | 0.66 ± 0.06a | 0.008 |
TRANSLATION | ||||
Ribosome | 2.41 ± 0.03b | 2.09 ± 0.03a | 2.32 ± 0.04b | < 0.001 |
Ribosome Biogenesis | 1.44 ± 0.04b | 1.31 ± 0.01a | 1.43 ± 0.01b | 0.002 |
Aminoacyl-tRNA biosynthesis | 1.25 ± 0.02b | 1.11 ± 0.02a | 1.19 ± 0.03b | 0.001 |
NUCLEOTIDE METABOLISM | ||||
Purine metabolism | 2.40 ± 0.05b | 2.24 ± 0.03a | 2.40 ± 0.02b | 0.007 |
Pyrimidine metabolism | 1.84 ± 0.05b | 1.60 ± 0.02a | 1.79 ± 0.02b | 0.001 |
Values within a column followed by different superscript letters differ significantly (P < 0.05). CTL, non-challenge control; CP, C. perfringens-challenged group; ARGCP, C. perfringens-challenged group fed diet supplemented with 0.3% l-arginine. Data are shown as the mean ± standard error.