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. 2018 Jul 1;29(13):1664–1674. doi: 10.1091/mbc.E18-02-0129

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© 2018 Elmore et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — The centrosomal targeting information of CK1δ/ε is located within the kinase domain. RPE-1 cells were transiently transfected with GFP N-terminal fusions to full length or truncations of CK1δ and CK1ε. Cells were fixed with 100% methanol and stained with γ-tubulin (magenta) and DAPI (blue). (A) Localization of full-length protein and truncation mutants in RPE-1 cells. (B) Quantification of the colocalization between γ-tubulin and full-length proteins or truncation mutants. (C) Localization of full-length GFP-CK1δ and GFP-CK1ε catalytically inactive mutants. (D, E) Quantification of the colocalization between γ-tubulin and GFP-CK1δ (D) or GFP-CK1ε (E) wild type or catalytically inactive mutants. For B, D, and E, 100 cells per experiment. n = 3. *, p < 0.05, **, p < 0.01, ***, p < 0.005, ****, p < 0.001, p values determined using ANOVA; ns, not significant. Error bars represent SEM. Scale bars: 15 μm.