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. 2018 Jul 1;29(13):1533–1541. doi: 10.1091/mbc.E18-01-0057

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© 2018 Muroyama et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Characterization of intestine-specific Nezha/CAMSAP3 deletion in the intestinal epithelium. (A) Alleles used to generate Nezha/CAMSAP3 deletion in the intestinal epithelium. (B) Western blot showing Nezha depletion in Villin-Cre Nezhafl/fl intestinal epithelia. (C) Nezha staining in control and Villin-Cre Nezhafl/fl crypts. Scale = 20 µm. (D) Nezha staining in control and Villin-Cre Nezhafl/fl villi. Scale = 20 µm. (E) Microtubule organization in control and Villin-Cre Nezhafl/fl villar and crypt cells. Scale = 10 µm. (F) Kaplan-Meier plot of indicated genotypes. (G) Weights of indicated genotypes at weaning. n.s. = not significant.