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. 2018 Jul 1;29(13):1611–1627. doi: 10.1091/mbc.E18-02-0088

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© 2018 Copeland et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 10: — FHDC1 overexpression disrupts the centriole maturation cycle. FHDC1 or FHDC1.I180A was expressed by transient transfection, and the effects on centrosome morphology and Cep170 localization were assessed by immunofluorescence. (A) mCherry (red) expression does not affect γ-tubulin staining (white), nor does it affect the number of Cep170 puncta detected with anti-Cep170 antibody (green). (B, C) FHDC1 overexpression induces the accumulation of supernumerary Cep170 puncta in both ciliated and nonciliated cells. (D) FHDC1.I180A overexpression does not affect centrosome morphology. (E) Quantification of data shown in A–D. N = 3, >100 cells counted per sample. Error bars = SEM. (F) mCherry (red) expression does not affect centriole number nor the distance between centrioles as detected by γ-tubulin staining (green). (G, H) FHDC1 (red) overexpression induces centriole separation and supernumerary centrioles in both ciliated and nonciliated cells. (I) FHDC1.I180A (red) overexpression does not affect centriole number or their separation morphology. (J) Quantification of data shown in F–I. N = 3, >100 cells counted per sample. Error bars = SEM.