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. 2018 Jul 1;29(13):1611–1627. doi: 10.1091/mbc.E18-02-0088

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© 2018 Copeland et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 11: — FHDC1-induced centriole separation is F-actin dependent. mCherry, FHDC1, or FHDC1.I180A was expressed in NIH 3T3 cells by transient transfection, and the effects on centriole separation were assessed by IF. (A) Overexpression of mCherry (red) does not affect centriole (γ-tubulin, green) separation, and centriole localization is not affected by 2 h of treatment with the actin-disrupting drug LatB. (B) Overexpression of FHDC1.I180A (red) does not affect centriole separation, and centriole localization is not affected by 2 h of treatment with the actin-disrupting drug LatB. (C) Overexpression of FHDC1 induces centriole separation and this effect is reversed following treatment with LatB. (D) Quantification of data shown in A–C. N = 3, >100 cells counted per sample. Error bars = SEM.