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. 2018 Jul 1;29(13):1611–1627. doi: 10.1091/mbc.E18-02-0088

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© 2018 Copeland et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 12: — FHDC1-induced effects on cilia assembly are rescued by expression of a constitutively active derivative of PLK1. (A–C) mCherry was coexpressed in NIH 3T3 cells with wild-type PLK1 or kinase-dead (K82R) or constitutively active (T210D) PLK1 derivatives (cyan), and the effects on ciliogenesis were assessed by immunofluorescence. None of the coexpressed PLK1 derivatives have substantial effects on ciliogenesis in mCherry control ceclls. (D–F) FHDC1 was coexpressed with wild-type PLK1 or kinase-dead (K82R) or constitutively active (T210D) PLK1 (cyan), and the effects on ciliogenesis were assessed by IF. Coexpression of PLK1.T210D suppresses the effects of FHDC1 on cilia length and the number of ciliated cells. (G, H) Quantification of data shown in A–F. N = 3, >100 cells counted per sample. Error bars = SEM. *indicates p < 0.005’, **indicates p < 0.0001.