FIGURE 4:

FHDC1-induced Golgi dispersion does not inhibit ciliogenesis. Full-length FHDC1 and a series of FHDC1 C-terminal–deletion derivatives (red) were expressed in NIH 3T3 cells and the effects on Golgi dispersion and ciliogenesis were assessed by immunofluorescence. Primary cilia were detected with anti-acetylated tubulin antibody (white) and the Golgi was detected via coexpression of the Golgi marker GFP-GalT (green). (A) Expression of full-length FHDC1 induces Golgi dispersion in essentially all transfected cells. Cells with clearly dispersed Golgi were still able to assemble greatly elongated primary cilia. (B) Expression of FHDC1.1004N (codons 1–1004) induces Golgi dispersion in nearly all cells. Primary cilia were present in more than half of 1004N-expressing cells. (C) Expression of FHDC1.978N (codons 1–978) induced Golgi dispersion in the majority of transfected cells and had little effect on cilia length or number. (D–F) Quantification of data shown in A–C. N = 3, >100 cells counted per experiment, error bars = SEM. Red bar indicates mean cilia length. *indicates significant difference from FHDC1-expressing cells, p < 0.001.