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. 2018 Jul 1;29(13):1611–1627. doi: 10.1091/mbc.E18-02-0088

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© 2018 Copeland et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — F-actin is present in the cilia of FHDC1-overexpressing cells. FHDC1 and FHDC1.I180A were expressed in NIH 3T3 cells by transient transfection and the effects on F-actin accumulation (green) and cilia assembly (white) were assessed by immunofluorescence. mCherry was used as a control. (A) F-actin is not detected in cilia of mCherry-expressing cells. (B) FHDC1.I180A is recruited to the cilia. F-actin is not detected in cilia of FHDC1.I180A-overexpressing cells. (C, D) F-actin is readily detected by phalloidin staining in the elongated cilia of FHDC1-overexpressing cells.