FIGURE 7:

FHDC1 expression inhibits cilia disassembly. mCherry, FHDC1, or FHDC1.I180A was expressed in NIH 3T3 cells by transient transfection and the transfected cells were serum-starved to induce cilia assembly. At 48 h after transfection the cells were stimulated with complete medium for 2, 3, or 4 h and the effects on ciliogenesis were assessed by immunofluorescence. (A) Serum stimulation rapidly induced cilia disassembly in mCherry- and I180A-expressing cells. The number of FHDC1-expressing cells with cilia was not affected by serum stimulation. N = 3, >100 cells counted per experiment. Error bars = SEM. (B) The data in A were replotted as percentages of the unstimulated values to facilitate comparison of the relative effects of FHDC1 and FHDC1.I180A on cilia resorption. (C) Quantification of cilia length for the data shown in A. N = 3, >100 cilia measured per sample.