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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Nature. 2017 Apr 19;544(7651):503–507. doi: 10.1038/nature22063

Extended Data Figure 7. Cohesin islands in Ctcf Wapl DKO MEFs.

Extended Data Figure 7

Binding of CTCF, Nipbl and cohesin (Stag1 and Scc1) at the convergently transcribed genes Mier1 and Slc35d1 (a) and Mllt1 and Dnajc1 (b) was determined by ChIP-seq analysis in WT MEFs, Ctcf, Smc3 and Wapl KO MEFs and Ctcf Wapl DKO MEFs. c,d. Time course analysis of the appearance of cohesin islands upon Ctcf and Wapl deletion in Adeno-Cre infected MEFs. c, Scc1 accumulation in the 3’ region of the convergently transcribed Usp47 and Dkk3 genes at the indicated days after Adeno-Cre infection. d, Density profiles of Scc1 accumulation at convergently transcribed genes in response to Adeno-Cre infection. Scc1 binding was centered in the middle of the intervening region between gene pairs with a similarly strong transcription activity (TPM > 5).