Figure 10.

Methods for the fluorescent labeling of library members, enabling fluorescence polatization measurement procedures. For single-pharmacophore library members, hits can be resynthesized as fluorescently-labeled compounds, or on oligonucleotides (DNA or LNA) which carry the fluorophore on the same strand or on a complementary strand. Similarly, hits from dual-pharmacophore chemical libraries can be re-synthesized as fluorescent molecules (with a suitable linker connecting the two building blocks) or on complementary oligonucleotides (one of which is fluorescently labeled).