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. 2018 Jul 15;29(14):1732–1742. doi: 10.1091/mbc.E17-06-0422

FIGURE 4:

FIGURE 4:

Nanotopography modulates the distribution of BCR clusters. (A) TIRF image of an A20 B-cells with AF546-labeled BCR on ridges with a 5-µm spacing. (B) A merged TIRF/IRM image of the same cell. The green line was used to calculate the intensity profile in D. Scale bar: 5 µm. (C) Peak-to-mean ratio of BCR fluorescence intensity over the entire cell/surface contact zone and observation time for cells spread on flat surfaces and on widely spaced ridged surfaces. (p < 0.001 KS test; N = 13 for flat, N = 24 for 3-µm and N = 19 for 5-µm surfaces). (D) Fluorescence intensity profile of AF546-labeled BCR along the line shown in (B). The gray bars indicate the locations of the ridges. (E) Normalized histogram of BCR cluster locations with respect to the nearest ridge for 5-μm spaced ridges (N = 19). (F) Normalized histogram of BCR cluster locations with respect to the nearest ridge for 3-μm-spaced ridges (N = 7). (G) Normalized histogram of BCR cluster locations for a flat surface with respect to a line bisecting the cell in a chosen direction (N = 10).