Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 15;29(14):1732–1742. doi: 10.1091/mbc.E17-06-0422

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Ketchum et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 5: — Nanotopography influences intracellular calcium signaling. (A) Integrated, whole-cell fluorescence intensity of the calcium-sensitive dye OGB-1 for representative A20 cells on flat surfaces and on ridges with a 5-μm spacing. (B) Distribution of peak-to-peak intervals between adjacent oscillations in OGB-1 fluorescence intensity for A20 cells on flat substrates (N = 27) and on ridges with a 5-μm spacing (N = 17). The average interval is significantly different for the two substrates (p << 0.001 KS test). (C) Distribution of peak-to-peak intervals between adjacent oscillations in OGB-1 fluorescence intensity for cells on a flat substrate before (–LatA, N = 27) and after (+LatA, N = 20) addition of 100 nM latrunculin-A (p << 0.001 KS test). (D) Distribution of peak-to-peak intervals between adjacent oscillations in OGB-1 fluorescence intensity for cells on ridges with a 5-μm spacing before (–LatA, N = 14) and after (+LatA, N = 12) addition of 100 nM latrunculin-A (p << 0.001 KS test). (E) Representative time dependence of the integrated actin fluorescence intensity within an ROI of an EGFP-actin A20 cell before and after 1 µM thapsigargin addition on ridges with a 5-μm spacing. The arrow indicates the time of the addition. (F) Coefficient of variation for integrated EGFP-actin intensity in ROIs as described previously, before (–Thap) and after (+Thap) the addition of 1 µM thapsigargin (N = 21, N = 20, respectively, p < 0.01 KS test).