FIGURE 2:

Inhibiting vacuole acidification suppresses temperature-sensitive growth of fab1∆ yeast. Live-cell confocal fluorescence microscopy analysis of Sna3-GFP (A) and Sna3-pHluorin (B) in the indicated yeast strains. The vacuole membrane is stained with the lipophilic fluorescent dye, FM 4-64. Dashed lines represent cell outlines. Scale bar = 4 µm. (C) Cell growth assay of the indicated yeast strains grown on rich agar medium plates at 26°C or 37°C for 3 d. Shown is a representative assay of three independent experiments.