Fig E5.

Proportion of activated mast cells sensitized with plasma samples containing varying levels of P-sIgE and Ara h 2–specific IgE. The lower limit of specific IgE above which the mast cell activation assay is reliable was estimated for peanut-specific IgE (0.4 KU/L) and for Ara h 2-specific IgE (0.2 KU/L) by observing the beginning of the inflection point from a cubic spline with a λ value of 0.8 (where the reference line intersects the smoothed regression line). PA, Peanut allergic; PS, peanut sensitized tolerant; NA, nonsensitized nonallergic.