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(A) E12.5 pancreata cultured in vitro for 1 day and submitted to forskolin treatment activating the CFTR channels undergo lumen swelling. The lumen appears as a dark shadow, and some are highlighted with arrows (B). Ductal spheres generated from E12.5 pancreata swell upon treatment with forskolin, as compared to untreated controls. CFTR, cystic fibrosis transmembrane conductance regulator; E, embryonic day.
