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Effects of ozone on spleen granulocytes and MDSCs. Spleen cells, collected 24–72 h after exposure of CTL mice to air or ozone, were immunostained with antibodies to CD11b, Ly6G, and Ly6C and then analyzed by flow cytometry. One representative histogram from 3 to 4 mice/treatment group is shown. Upper panel: CD11b+ spleen cells expressing Ly6G. Lower panel: CD11b + Ly6G+ spleen cells expressing Ly6C.
