FIG. 5.

Effects of splenectomy on lung macrophages responding to ozone. BAL cells, collected 24–72 h after exposure of CTL and SPX mice to air or ozone, were stained with antibodies to CD11b, Ly6G, Ly6C, F4/80, and CD11c or isotypic controls and analyzed by flow cytometry. Macrophages (MP) were defined as CD11b ⁺ Ly6G⁻ F4/80 ⁺ CD11c⁺and Ly6C^Hi (pro-inflammatory) or Ly6C^Lo (anti-inflammatory). Bars, mean ± SE (n = 10 mice/treatment group). ^aSignificantly different (P < .05) from air-exposed animals; ^bSignificantly different (P < .05) from CTL.