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Effects of splenectomy on ozone-induced MMP-9 expression. Lung sections, prepared 24–72 h after exposure of CTL and SPX mice to air or ozone, were stained with antibody to MMP-9. Binding was visualized using a peroxidase DAB substrate kit. Arrows indicate alveolar macrophages. One representative lung section from 3 mice/treatment group is shown. Original magnification, 600×.
