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. 2001 Oct 30;98(23):12972–12977. doi: 10.1073/pnas.241286698

Figure 1.

Figure 1

A growth assay for the cricket IGR-IRES function in vivo. (A) A diagram of the dicistronic reporter construct that contains a CrPV IGR-IRES element preceding the second cistron. The expression of the reporter mRNA is directed by the copper promoter (Cup1). (B) Growth assay for yeast strains H1402 (isogenic wild type), H1692 (GCN2c), and H1613 (GCN2c) transformed with either pCup1 LEU2 IGR URA3 (IGR) or pCup1 LEU2 IGRmut14 URA3 (mut14) plasmids containing the dicistronic reporter. Transformants were streaked on minimal SD medium supplemented with 100 μM Cu2+, inositol, with or without uracil, as indicated.