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. 2001 Oct 30;98(23):12972–12977. doi: 10.1073/pnas.241286698

Figure 5.

Figure 5

IGR-IRES activity in yeast extracts. (A) Diagram of dicistronic mRNAs containing the Renilla luciferase (R-Luc) as the first cistron and the firefly luciferase (F-Luc) as the second cistron. (B) Genotype of the wild-type (IGR) and various mutant IGR-IRES elements. Nucleotide sequences that differ from the wild-type IGR are shown in bold and underlined. (C) Translation-competent yeast extracts were programmed with various dicistronic RNAs, and the ratios of F-Luc to R-Luc production are indicated. (D) The primary data are shown. Δ represents the dicistronic mRNA without any IGR-IRES sequences inserted. Standard error of three experiments is indicated.