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The in vitro translation activity of the wild-type CrPV IGR-IRES in the presence of edeine. Translation-competent yeast extract was preincubated for 5 min with the indicated concentrations of edeine and programmed with the IGR dicistronic mRNA diagramed in Fig. 5. Translation efficiencies of F-Luc (shaded) and R-Luc (unshaded) are shown. Error bars represent the standard error of three experiments.
