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. 2018 Aug 6;215(8):2055–2072. doi: 10.1084/jem.20172049

Figure 6.

Figure 6.

Eosinophils suppress C. rodentium–specific Th1 and Th17 responses and colitis. (A–H) PHIL mice and their WT littermates were infected with C. rodentium for 12 d. (A) Colonic LP eosinophil numbers of infected PHIL and WT relative to naive WT mice and their activation state. (B) Colonic neutrophil and CD4+ T cell numbers of the mice shown in A. (C) Th1 and Th17 cell frequencies in the colonic LP of C. rodentium–infected mice. (D) Cytokine and inflammatory gene expression in the colonic mucosa of C. rodentium–infected mice as determined by qRT-PCR. (E and F) C. rodentium–induced colitis as assessed by histopathological examination of H&E-stained sections. Representative sections of C. rodentium–infected WT and PHIL mice are shown in E, along with colitis scores in F. (G) C. rodentium colonization of the colons and cecums of the mice shown in A–E. (H) EPX expression in the colonic mucosa of the mice shown in A–G, as assessed by ELISA. Data in A–H are representative of two independent experiments. (I) CD63 expression as a marker of eosinophil degranulation, as assessed by FACS of colonic leukocyte preparations from the naive and C. rodentium–infected mice shown in A–H. (J and K) Eo-Cre+ × Ifngrfl/fl mice and their Cre-negative (WT) littermates were infected with C. rodentium for 12 d and assessed with respect to their colonization levels (J) and EPX expression in the colon as assessed by ELISA (K). Bar, 100 µm. *, P < 0.05; **, P < 0.01; ***, P < 0.001, as calculated by Mann-Whitney test.