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. 2018 Aug 6;215(8):2073–2095. doi: 10.1084/jem.20180010

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© 2018 Avery et al.

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Figure 8. — Pik3cd GOF B cells show defective switching but normal expansion and affinity maturation in vivo. WT or Pik3cd^GOF SWHEL cells were transferred to WT congenic hosts, which were then immunized with HEL-2x-SRBC. (A) The expansion of SW_HEL cells was tracked over time (mean ± SEM, n = 3–4 mice per group, representative experiment shown). (B) Percentage of cells with a plasmablast or GC phenotype was determined. (C) Percentage of cells that switched to IgG1 or were unswitched (IgM⁺) was determined in the plasmablast and GC populations. (D) Levels of HEL-specific serum Ig of various classes at day 5.5 were determined by ELISA. (B–D, each linked point shows mean ± SEM [n = 3–5] of single experiment). (E) Donor GC IgG1⁺ cells were sorted on day 10 and sequenced to identify mutations. Significant differences were determined by paired t tests. **, P < 0.01.