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. 2018 Aug 6;215(8):2137–2155. doi: 10.1084/jem.20171066

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© 2018 Stolte et al.

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Figure 5. — Validation of PPM1D and USP7 as dependencies in TP53 wild-type Ewing sarcoma. (A) Western blots showing decreased protein levels of USP7 after infection with sgRNAs targeting USP7 compared with control sgRNAs. (B) Relative viability of Ewing sarcoma cells infected with sgRNAs targeting USP7 or control sgRNAs 14 d after infection. Each data point shows the mean of eight replicates; data are plotted as mean values ± standard deviation. The experiment was performed twice and data points of one representative experiment are shown. (C) Western blots showing decreased protein levels of Wip1 after infection with sgRNAs targeting PPM1D compared with control sgRNAs. (D) Relative viability of Ewing sarcoma cells infected with sgRNAs targeting PPM1D or control sgRNAs 14 d after infection. Each data point shows the mean of eight replicates, and data are plotted as mean values ± standard deviation. The experiment was performed twice and data points of one representative experiment are shown. Significance was calculated by paired, two-tailed t test: not significant (n.s.) for P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.