Figure 2.

DCAF2 in dendritic cells is required for T cell homeostasis. (A) IB analysis of DCAF2 using lyses of BMDCs, T, and B cells from DCAF2^DKO mice (n = 3/group). (B) Cell cycle process of T cells or CD11c⁺ DCs from WT and DCAF2^DKO mice (n = 3/group) was evaluated by the DNA synthesis in vivo. (C) Flow cytometric analyses of the subpopulation frequencies of dendritic cells in Spl or iLN from 6-wk-old WT and DCAF2^DKO mice (n = 3/group). (D) The absolute cell numbers of CD4⁺, CD8⁺ T cells in Spl or iLN. (E) Flow cytometric analysis of the percentage of IFN-γ– and IL-17–producing CD4⁺ T cells in the Spl of 6-mo-old WT and DCAF2^DKO mice (n = 3/group). (F) H&E staining of the indicated tissue sections from 6-mo-old WT and DCAF2^DKO mice (n = 3/group), showing immune cell infiltrations (arrows). Inflammation score was quantified by a pathologist blinded to the groups. Bars, 200 µm. All FACS data are presented as a representative plot and summary graph of subpopulation percentage. All data are representative of three independent experiments. Error bars show mean ± SEM. Significance was determined by two-tailed Student’s t test. *, P < 0.05; ***, P < 0.005.