Table 2. Enriched gene sets within up-regulated in Nifedipine-treated HSCs, as compared to HSCs cultured under control conditions.

Gene set name	Systematic name	NES	P value	Q value
HALLMARK_P53_PATHWAY	M5939	2.00	<0.01	0.019
HALLMARK_INFLAMMATORY_RESPONSE	M5932	1.98	<0.01	0.019
HALLMARK_APOPTOSIS	M5902	1.90	<0.01	0.027
HALLMARK_HYPOXIA	M5891	1.86	<0.01	0.025
HALLMARK_IL2_STAT5_SIGNALING	M5947	1.83	<0.01	0.028
HALLMARK_KRAS_SIGNALING_UP	M5953	1.82	<0.01	0.026
HALLMARK_UV_RESPONSE_DN	M5942	1.80	<0.01	0.025
HALLMARK_INTERFERON_ALPHA_RESPONSE	M5911	1.78	<0.01	0.025
HALLMARK_INTERFERON_GAMMA_RESPONSE	M5913	1.76	<0.01	0.024
HALLMARK_NOTCH_SIGNALING	M5903	1.72	<0.01	0.029
HALLMARK_IL6_JAK_STAT3_SIGNALING	M5897	1.72	<0.01	0.028
HALLMARK_MYOGENESIS	M5909	1.58	<0.01	0.044
HALLMARK_HEME_METABOLISM	M5945	1.55	<0.01	0.054
HALLMARK_TNFA_SIGNALING_VIA_NFKB	M5890	1.54	<0.01	0.053
HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION	M5930	1.51	<0.01	0.062
HALLMARK_APICAL_SURFACE	M5916	1.40	0.042	0.098
HALLMARK_ESTROGEN_RESPONSE_LATE	M5907	1.38	0.05	0.106

The transcriptome data of three-cell-divided HSCs cultured with or without Nifedipine were subjected to GSEA, and the threshold was set at P value < 0.05. The normalized enrichment score (NES) reflects the degree to which a gene set is overrepresented at the top or bottom of a ranked list of genes. Bold indicates the gene sets in which q < 0.05.