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. 2018 Aug 6;217(8):2727–2742. doi: 10.1083/jcb.201712113

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© 2018 Hung et al.

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Figure 5. — Secreted factors are not required for down-regulation of dendritic growth by TLR8 activation. (A and B) The effects of TLR7 and TLR8 activation for 6 h on cytokine and chemokine expressions of cultured neurons. Q-PCR was performed by normalization with internal control Gm10069 or Gapdh as indicated. Three embryonic cortices and hippocampi of the same genotype were combined for one set of experiments. One dot indicates the data of one independent experiment. Data were collected from six experiments (n = 6). (C and D) Experiments of conditioned medium (CM). Experimental flowcharts are summarized in the left panel. The effects of conditioned medium on dendritic length are summarized in the right panel. The sample size (n) indicates the number of examined neurons randomly collected blind from two (D) or three (C) independent experiments. The data are presented as mean + SEM (error bars). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Two-way (A) and one-way (B) ANOVA with Bonferroni’s multiple comparisons test as well as two-tailed nonparametric Mann–Whitney tests (C and D) were used.