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. 2018 Aug 6;217(8):2743–2763. doi: 10.1083/jcb.201710116

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© 2018 Gómez-Sánchez et al.

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Figure 5. — Atg2 and Atg2^PM1 bind membranes similarly. (A) Atg2-mGFP isolated from WT or atg18Δ cells was incubated with GUVs as in Fig. 4 A. GUVs without PtdIns3P were used as controls. Binding to GUVs was quantified as described in Materials and methods. (B) Purified and DY-647–labeled Atg2 or Atg2^PM1 were incubated with GUVs, and binding was quantified and controlled as in A. At least 30 GUVs per sample were counted, and graphs represent means of three independent experiments ± SD. Bars, 10 µm. MW, molecular weight.